Playing Snake With Digital Microfluidics

July 12, 2025 by 2 Comments

Display technology has come a long way since the advent of the CRT in the late 1800s (yes, really!). Since then, we’ve enjoyed the Nixie tubes, flip dots, gas plasma, LCD, LED, ePaper, the list goes on. Now, there’s a new kid on the block — water.

[Steve Mould] recently got his hands on an OpenDrop — an open-source digital microfluidics platform for biology research. It’s essentially a grid of electrodes coated in a dielectric. Water sits atop this insulating layer, and due to its polarized nature, droplets can be moved around the grid by voltages applied to the electrodes. The original intent was to automate experiments (see 8:19 in the video below for some wild examples), but [Steve] had far more important uses in mind.

When [Steve]’s €1,000 device shipped from Switzerland, it was destined for greatness. It was turned into a game console for classics such as Pac-Man, Frogger, and of course, Snake. With help from the OpenDrop’s inventor (and Copilot), he built paired-down versions of the games that could run on the 8×14 “pixel” grid. Pac-Man in particular proved difficult, because due to the conservation of mass, whenever Pac-Man ate a ghost, he grew and eventually became unwieldy. Fortunately, Snake is one of the few videogames that actually respects the laws of classical mechanics, as the snake grows by one unit each time it consumes food.

[Steve] has also issued a challenge — if you code up another game, he’ll run it on his OpenDrop. He’s even offering a prize for the first working Tetris implementation, so be sure to check out his source code linked in the video description as a starting point. We’ve seen Tetris on oscilloscopes and 3D LED matrices before, so it’s about time we get a watery implementation.

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On-Site Viral RNA Detection In Wastewater With Paper And Wax Microfluidics

October 9, 2024 by 4 Comments
Schematic version of on-site wastewater analysis using the microfluidic strips (Credit: Yuwei Pan et al., Cell, 2024)

Wastewater sampling has become a popular way over the years to keep track of the health of a population, including human ones, as pathogens are often detectable in the effluence from toilets. Since most houses connected to the centralized sewer systems, this means that a few sampling sites suffice to keep tabs on which viruses are circulating in an area. While sampling this wastewater is easy, the actual RNA analysis using PCR (polymerase chain reaction) still has to be performed in laboratories, adding complex logistics. An approach for on-site analysis using microfluidics was tested out by [Yuwei Pan] et al., as recently published in Cell.

This particular approach uses RT-LAMP (reverse-transcription loop-mediated isothermal amplification) to increase the amount of genetic material, which has the significant benefit over PCR that it does not require multiple thermal cycles, instead being run at a constant temperature. The filter paper used as the basis has wax microchannels printed on it, which help to guide the filtered wastewater to the reaction chambers. This is in many ways reminiscent of the all too familiar linear flow self-tests (RAT: rapid antigen test) that have become one of the hallmarks of the SARS-CoV-2 pandemic.

What this paper microfluidic device adds is that it doesn’t merely contain antigens, but performs the lysis (i.e. breakdown of the virus particles), genetic material multiplication using RT-LAMP and subsequent presence detection of certain RNA sequences to ascertain the presence of specific viruses. Having been used in the field already since 2020 in the UK, the researchers envision this type of on-site analysis to be combined with a smartphone for instant recording and transmission to health authorities.

Some of the benefits of this approach would be lower cost, easier logistics and faster results compared to shipping wastewater samples to central laboratories.

Discount Microfluidics From A $9 Spree At The Dollar Store

July 11, 2021 by 1 Comment

Microfluidics — working with tiny volumes of fluids in tiny channels — isn’t something you’d think would be inexpensive. Unless you read [Alexander Bissells’] post on how he created microfluidic devices using stuff from the dollar store. The channels in these devices can be much smaller than a millimeter and the fluid volumes are sometimes measured in femtoliters. At those scales, fluids don’t work like we intuitively think they will.

The parts list included gel tape, baby droppers, and some assorted containers and tools. Total price at the dollar store $9. One of the key finds in the dollar store was some small spray bottles. They weren’t important themselves, but they contain small lengths of silicone tubing and that was useful. Plastic fresnel lenses along with the tubing and gel tape worked to make “chips.” The gel tape also gets cut to make the channels. An eyedropper with some modifications makes a reasonable syringe.

We aren’t sure what you can practically do with any of these, but the T-junction looked pretty interesting. If you want some ideas on how these devices work in biology, including COVID-19 testing, check out this article. And just last week [Krishna Sanka] hosted a Hack Chat on microfluidics in biohacking, you can find the transcript on the project page. If you need a pump, this one uses 3D printer firmware to control it.

Microfluidics For Biohacking Hack Chat

July 5, 2021 by 2 Comments

Join us on Wednesday, July 7 at noon Pacific for the Microfluidics for Biohacking Hack Chat with Krishna Sanka!

“Microfluidics” sounds like a weird and wonderful field, but one that doesn’t touch regular life too much. But consider that each time you fire up an ink-jet printer, you’re putting microfluidics to work, as nanoliter-sized droplets of ink are spewed across space to impact your paper at exactly the right spot.

Ink-jets may be mundane, but the principles behind them are anything but. Microfluidic mechanisms have found their way into all sorts of products and processes, with perhaps the most interesting uses being leveraged to explore and exploit the microscopic realms of life. Microfluidics can be used to recreate some of the nanoscale biochemical reactions that go on in cells, and offer not only new ways to observe the biological world, but often to manipulate it. Microfluidics devices range from “DNA chips” that can rapidly screen drug candidates against thousands of targets, to devices that can rapidly screen clinical samples for exposure to toxins or pathogens.

There are a host of applications of microfluidics in biohacking, and Krishna Sanka is actively working to integrate the two fields. As an engineering graduate student, his focus is open-source, DIY microfluidics that can help biohackers up their game, and he’ll stop by the Hack Chat to run us through the basics. Come with your questions about how — and why — to build your own microfluidics devices, and find out how modern biohackers are learning to “go with the flow.”

join-hack-chatOur Hack Chats are live community events in the Hackaday.io Hack Chat group messaging. This week we’ll be sitting down on Wednesday, July 7 at 12:00 PM Pacific time. If time zones have you tied up, we have a handy time zone converter.

[Featured image: Cooksey/NIST]

This Camera Captures Piezo Inkjet Micro-Drops For DIY Microfluidics

April 15, 2020 by 6 Comments

In microfluidics, there are “drop on demand” instruments to precisely deposit extremely small volumes (pico- or nano-liters) of fluid. These devices are prohibitively expensive, so [Kyle] set out to design a system using hobbyist-level parts for under $1000. As part of this, he has a fascinating use case for a specialized camera: capturing the formation and shape of a micro-drop as it is made.

There are so many different parts to this effort that it’s all worth a read, but the two big design elements come down to:

  1. Making the microdrop using a piezo element
  2. Ensuring the drop is made correctly, and visually troubleshooting
Working prototype. The piezo tube is inside the blue piece at the top. The camera is to the right, and the LED strobe is on the left.

It’s one thing to make an inkjet element in a printer work, but it’s quite another to make a piezoelectric element dispense arbitrary liquids in a controlled, repeatable, and predictable way. Because piezoelectric elements force liquid out with a mechanical motion, different liquids require different drive signals and that kind of experimentation requires a way to see what is going on, hence the need for a drop observation camera.

[Kyle] ended up taking the lens assembly from a cheap USB microscope and mating it to his Korukesu C1 USB Camera with a 3D printed assembly. Another 3D printed enclosure doubles as a lightbox, holding the piezo tube in the center with the LED strobe and camera on opposite sides. The whole assembly had a few false starts, but in the end [Kyle] seems pretty happy with his results. The device is briefly described at a high level here. There are some rough edges, but it’s a working system.

Inkjet technology has been around for a long time (you can see a thirty-plus year old inkjet printer in action here) but it’s worth mentioning that not all inkjet heads are alike. Most inkjet printer heads operate thermally, which means a flash of heat vaporizes some ink to expel a micro-drop. These heads aren’t very suitable for microfluidics because not only do they rely on vaporizing the liquid, but they also don’t work well with anything other than the ink they’re designed for. Piezoelectric print heads are less common, but are more suited to the kind of work [Kyle] is doing.

Norovirus Smartphone: Using Megapixels And Microfluidics To Fight The Other Kind Of Virus Infection

September 10, 2019 by 8 Comments

Chances are pretty good that at some time in your life, you’ve crossed paths with a norovirus. And chances are that you remember the encounter vividly, or at least its aftermath. I recall a run-in with the bug one Christmas, when my parents brought over more than just toys for the kids when they visited. Within a day, everyone in the house was sharing the joy. Twas the season; they don’t call it the winter vomiting bug for nothing.

Most of the 685 million norovirus infections each year resolve after a few miserable days, but some require hospitalization and 200,000 of them result in death, mainly from dehydration and mainly children. An easy to use, cheap, and accurate means of detecting the virus in the field would be quite a boon to public health. And soon, smartphones may be able to do just that.

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Making Microfluidics Simpler With Shrinky Dinks

April 23, 2019 by 6 Comments

It’s as if the go-to analogy these days for anything technical is, “It’s like a series of tubes.” Explanations thus based work better for some things than others, and even when the comparison is apt from a physics standpoint it often breaks down in the details. With microfluidics, the analogy is perfect because it literally is a series of tubes, which properly arranged and filled with liquids or gasses can perform some of the same control functions that electronics can, and some that it can’t.

But exploring microfluidics can be tough, what with the need to machine tiny passages for fluids to flow. Luckily, [Justin] has turned the process into child’s play with these microfluidic elements made from Shrinky Dinks. For those unfamiliar with this product, which was advertised incessantly on Saturday morning cartoon shows, Shrinky Dinks are just sheets of polystyrene film that can be decorated with markers. When placed in a low oven, the film shrinks about three times in length and width while expanding to about nine times its pre-shrunk thickness. [Justin] capitalized on this by CNC machining fine grooves into the film which become deeper after shrinking. Microfluidics circuits can be built up from multiple layers. The video below shows a mixer and a simple cell sorter, as well as a Tesla valve, which is a little like a diode.

We find [Justin]’s Shrinky Dink microfluidics intriguing and can’t wait to see what kind of useful devices he comes up with. He’s got a lot going on, though, from spider-powered beer to desktop radio telescopes. And we wonder how this technique might help with his CNC-machined microstrip bandpass filters.

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